Probably to become with out Lucas Biglia, Lazio are preparing for any challenging test at Bayer Leverkusen.
Lazio coach Stefano Pioli was left hopeful over Lucas Biglia's injury as he looked ahead to Wednesday's Champions League clash against Bayer Leverkusen.
Biglia, a reported transfer target for Manchester United, came off inside the 52nd minute of Lazio's 2-1 Serie A win over Bologna on Saturday.
The club confirmed Biglia, who opened the scoring in the victory, had suffered a calf injury that would call for additional tests to figure out its severity.
Pioli was hopeful as Lazio prepare to head to Germany for the play-off getting won the initial leg 1-0.
"Our subsequent game is hugely essential as it'll tell us which European competitors we are going to take component in," he told Sky Sport Italia.
"The team is competitive. Sadly, we had a handful of also lots of injuries and hope Lucas Biglia doesn't have something critical."
Leverkusen may also take winning form in to the second leg right after overcoming Hannover 1-0 within the Bundesliga on Saturday.
Meanwhile, Monaco welcome Valencia to the Stade Louis II with the La Liga outfit holding a 3-1 advantage.
Both failed to win league matches on Saturday as Monaco have been held at Toulouse and Valencia drew at Rayo Vallecano.
Manchester United are well-placed to attain the group stages as they head to Club Brugge also top 3-1.
Marouane Fellaini's late aim at Old Trafford has given them a two-goal buffer, but Louis van Gaal's guys are struggling for ambitions - having scored just twice in 3 league games this season.
There remains higher uncertainty about all the other ties.
Leigh Griffiths' brace helped Celtic beat Malmo, however the Swedish side host the second leg right after the 3-2 loss in Scotland.
Shakhtar Donetsk will fancy their chances of reaching the group stages, top Speedy Vienna 1-0 ahead with the second leg in Ukraine.
Basel take a look at Israel's Maccabi Tel Aviv right after a 2-2 draw in Switzerland, when Astana head to APOEL holding a 1-0 lead.
CSKA Moscow are trailing 2-1 ahead of hosting Sporting Lisbon and BATE take a 1-0 bring about Partizan Belgrade.
Croatian club Dinamo Zagreb hold the upper-hand in their tie against Skenderbeu Korce, obtaining won the initial leg 2-1 in Albania.